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NIKON

ECLIPSE Ni-E/Ni-U

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Upright Microscope
ECLIPSE Ni-E/Ni-U

 

  • Stratum structure improves system expandability
  • High-speed motorized accessories
  • Two exchangeable focusing mechanisms
  • Automatic adjustment with objective changeover
  • Change of observation conditions
  • High-precision motorized focusing
  • Supreme optical performance
  • Ultimate ease of operation


Ni-E (focusing stage) configured with motorized epi-fluorescence illuminator, motorized condenser and motorized quadrocular tilting tube


Ni-E (focusing nosepiece) configured with motorized excitation and barrier filter wheels, motorized epi-fluorescence illuminator, confocal microscope system, high-sensitivity camera, motorized stage and motorized quadrocular tilting tube

ECLIPSE Ni-E, Nikon’s top-of-the-line motorized upright microscope, was developed to meet the increasing demands for sophistication and automation of research. ECLIPSE Ni-E boasts flexible system expandability and supports a broad range of advanced research applications.
With Nikon’s proprietary stratum structure, exchangeable focusing mechanisms, from focusing stage to focusing nosepiece, a diverse array of motorized accessories, and automatically changing observation conditions, Ni-E has expanded application possibilities to provide the perfect solution for all advanced bioscience and medical research.

Stratum structure improves system expandability

Nikon’s proprietary stratum structure enables simultaneous mounting of two optical paths on one microscope to support various applications.
This structure allows double layer mounting of an epi-fluorescence illuminator and a barrier filter wheel, or a photoactivation unit and a back port unit, developed for the first time for upright microscopes.


Ni-E (focusing nosepiece) configured with back port unit, motorized epi-fluorescence illuminator, two high-sensitivity cameras, motorized stage and motorized quadrocular tilting tube


Ni-E (focusing nosepiece) configured with photoactivation unit, motorized epi-fluorescence illuminator, high-sensitivity camera, motorized stage and motorized quadrocular tilting tube

High-speed motorized accessories

The wide array of motorized accessories clearly differentiated by function, including the newly developed motorized excitation filter wheel and motorized barrier filter wheel, can be custom-combined depending on application.

Two exchangeable focusing mechanisms


Ni-E (focusing nosepiece) configured with multiphoton confocal microscope system, episcopic and diascopic NDD units, motorized epi-fluorescence illuminator and motorized quadrocular tilting tube

The microscope can be modified by switching the focusing stage and focusing nosepiece, enabling a fixed-stage configuration with motorized focusing to meet the demands of experiments such as in vivo deep imaging utilizing multiphoton confocal microscopy.

Automatic adjustment with objective changeover

Condenser, aperture, field diaphragm and ND filter are automatically set to optimal positions according to the objective magnification in use. In addition, XYZ stage travel distance per handle rotation and parfocal distance deviation correction are automatically adjusted. Microscope settings can also be manually adjusted.

Change of observation conditions

Selected observation conditions can be designated to individual buttons, enabling changes to be made at the push of a button. This is particularly convenient when reproducing specific observation conditions.

High-precision motorized focusing

High-precision Z-focus provides accurate Z-position information required for use with confocal laser microscopes and supports automated Z-series acquisition. Individual coarse and fine focus knobs enhance ease of operation.

Supreme optical performance

Nano Crystal Coat technology

Employed for the first time in microscope objectives, this anti-reflective coating consists of nanometer-size particles. It is based on semiconductor manufacturing technology and is also used for Nikon camera lenses. The coating’s coarse structure and the arrangement of particles in a spongy construction with uniform spaces between them achieve extremely low refractive indices.

CFI Plan Apochromat λ series objective lens

With remarkably high NA, greatly improved transmission in the long wavelength range thanks to Nikon proprietary Nano Crystal Coat, and chromatic aberration correction from 435 nm to 850 nm, these objectives are ideally suited not only for brightfield and DIC observations but also for fluorescent observations. These lenses allow bright and clear image acquisition at any wavelength for near-IR imaging and multi-color fluorescence imaging. Because bright images can be captured even with a weak excitation light, damage to specimens is minimized.

Water dipping objective lenses

With a long working distance and high NA, these objectives provide excellent transmission in near IR wavelength range. The axial chromatic aberration of 40x and 60x objectives has been corrected to up to 850 nm, allowing high-resolution images of minute structures in thick specimens during IR-DIC observation. The 25xW MP and 100x objectives feature high NA and a long working distance. With chromatic aberration corrected in the IR region, these objectives are ideal for multiphoton excitation observation. In addition, by employing a mechanism to compensate for the changes in spherical aberration that occur at different temperatures and depths of observation points, clear images of areas deep within thick specimens can be captured.

Uniformly bright illumination

The“fly-eye”lens is ideally suited to diascopic illumination optical systems. Uniform and bright illumination up to the viewfield periphery is provided at any magnification.

Fluorescence noise elimination

Nikon’s proprietary noise terminator mechanism is employed in the epi-fluorescence cube turret and filter cubes. The S/N ratio has been dramatically improved by thoroughly eliminating stray light in the filter cubes, allowing images of weak fluorescent signals to be captured with high contrast and brightness.

Ultimate ease of operation

Simple digital imaging

Images can be captured with Digital Sight cameras by simply pressing the image capture button located on the microscope base.

3D ergo design

Most microscope controls can be operated with the easy-to-reach buttons on the front of the Ni-E. Operation buttons on the microscope’s sides are angled to allow intuitive touch-type operations during observation.

Controllers supporting intuitive operations

The DS-L3 stand-alone control unit allows Ni-E microscope functions, a Digital Sight camera and peripheral devices to be controlled using a mouse or a touch panel without a PC connection. An ergo controller with a similar operational feel as that of an actual microscope is also available.

Stand-alone control unit DS-L3

 Ni-ENi-U
Focusing stage typeFocusing nosepiece type 
Main bodyOptical systemCFI60 infinity optical systemCFI60 and CFI75 infinity optical systemsCFI60 infinity optical system
Focusing
(stroke from focus point)
Via motorized stage Up/Down movement
(Up 2 mm/Down 13 mm)
Via motorized nosepiece Up/Down movement
(Up 13 mm/Down 2 mm)
Via manual stage Up/Down movement
(Up 3 mm/Down 26 mm)
Built-in linear encoder, Resolution: 0.025 µm
Motorized escape and refocus mechanism
Coaxial Coarse/Fine focusing
Illumination
  • Halogen lamp (12V100W)
  • NI-ND-E Motorized ND Filter (option)
Halogen lamp (12V100W)
Built-in fly-eye lens
Built-in NCB11, ND8, ND32 filters (detachable, one additional filter mountable) and diffuser (non-detachable)
ND2 filter (option)
ControlsTransmitted light On/Off switch, Intensity control dial with preset function
Image capture button
Built-in motorized control switches
  • Stand-alone control unit DS-L3
  • NI-ERG NI Ergo Controller (option)
  • Stand-alone control unit DS-L3 (option)
  • NI-SRCP Simple Remote Control Pad (option)
Power supply unitExternal power supply NI-CTLA Control Box A for all configurationsBuilt-in for halogen lamp
NI-CTLB Control Box B is necessary when Motorized/Intelligent options are combined.
Eyepieces (F.O.V. mm)
  • CFI 10x (22)
  • CFI 10xM Photomask (22)
  • CFI 12.5x (16)
  • CFI 15x (14.5)
  • CFI UW10x (25)
  • CFI UW10xM Photomask (25)
TubesF.O.V. 22 mm
(Eyepiece/Port)
  • C-TB Binocular Tube
  • C-TE2 Ergonomic Binocular Tube (100/0, 50/50 with C-TEP2 DSC Port)
    Inclination angle: 10-30 degree, Extension up to 40 mm
F.O.V. 25 mm*1
(Eyepiece/Port)
  • C-TF Trinocular Tube F (100/0, 0/100)
  • C-TT Trinocular Tube T (100/0, 20/80, 0/100)
  • C-TT-C Trinocular Tube (100/0, 0/100, for confocal)*2
  • NI-TT Quadrocular Tilting Tube (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100)
    Inclination angle: 15-35 degree
  • NI-TT-E Motorized Quadrocular Tilting Tube
    (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100)
    Inclination angle: 15-35 degree
Ports (F.O.V. 11 mm)· C-TEP2 DSC Port for Ergonomic Binocular (with C-mount adapter, 0.7x)
· NI-BPU Back Port Unit (with C-mount adapter, 1.0x)
· NI-RPZ DSC Zooming Port for Quadrocular Tube (with C-mount adapter, manual zoom, 0.6x - 2.0x)
· NI-RPZ-E Motorized DSC Zooming Port for Quadrocular Tube (with C-mount adapter, motorized zoom, 0.6x - 2.0x)
Arms
  • NI-SAM Standard Arm
  • NIE-CAM Contact Arm (for Motorized/Intelligent options)
  • NIU-CAM Contact Arm (for Motorized/Intelligent options)
NosepiecesMotorized
  • NI-N7-E Motorized Septuple Nosepiece
  • NI-ND6-E Motorized Sextuple DIC Nosepiece
  • NI-N7-E Motorized Septuple Nosepiece
  • NI-ND6-E Motorized Sextuple DIC Nosepiece
Intelligent
  • NI-N7-I Intelligent Septuple Nosepiece
  • NI-ND6-I Intelligent Sextuple DIC Nosepiece
  • NI-N7-I Intelligent Septuple Nosepiece
  • NI-ND6-I Intelligent Sextuple DIC Nosepiece
Manual
  • D-ND6 Sextuple DIC Nosepiece
  • C-N Sextuple Nosepiece
  • C-NA Sextuple Nosepiece with Analyzer Slot
  • L-NU5 Universal Quintuple Nosepiece ESD
  • L-NBD5 BD Quintuple Nosepiece ESD
  • FN-S2N Sliding Nosepiece
    (for CFI60 objectives)
    Changeover 2 objectives, DIC slider insertable
  • FN-MN-H Single Objective Holder
    (for CFI75 objective)
    DIC slider insertable
  • D-ND6 Sextuple DIC Nosepiece
  • C-N Sextuple Nosepiece
  • C-NA Sextuple Nosepiece with Analyzer Slot
  • L-NU5 Universal Quintuple Nosepiece ESD
  • L-NBD5 BD Quintuple Nosepiece ESD
Stages
  • NIE-CSRR2 Right Handle Rotatable Ceramic-coated Stage with 2S Holder
    Cross travel 78(X) x 54(Y) mm
    Handle height and torque adjustable
  • FN-3PS2 FN1 Standard Stage
    Cross travel 30(X) x 30(Y) mm
  • C-SR2S Right Handle Stage with 2S Holder
  • C-CSR1S Right Handle Ceramic-coated Stage with 1S Holder
  • C-CSR Right Handle Ceramic-coated Stage
  • NIU-CSRR2 Right Handle Rotatable Ceramic-coated Stage with 2S Holder
    Cross travel 78(X) x 54(Y) mm
    Handle height and torque adjustable
  • NI-S-E Motorized XY Stage
    Resolution: 0.1 µm
  • NI-SH-D Dish Holder (option)
Substages
  • NI-SSR Substage
    (for Motorized Universal Condenser and Rotatable / Motorized stages)
  • NI-SSF Substage for Focusing Nosepiece
    (for LWD condenser and FN1 Standard / Motorized stages)
  • NI-SSR Substage (for Rotatable stage)
  • NI-SS Substage (for Non-rotatable stages)
CondensersMotorized
  • NI-CUD-E Motorized Universal Condenser Dry
    For DIC, phase contrast, darkfield observations
    Attached on NI-SSR Substage
Manual
  • NI-CUD Universal Condenser Dry
  • C-C Abbe Condenser NA 0.9
  • C-C Achromat Condenser NA 0.9
  • Darkfield Condenser (Oil)
  • Darkfield Condenser (Dry)
  • C-C Achromat / Aplanat Condenser NA 1.4
  • C-C Slide Achromat Condenser 2-100x
  • C-C Achromat Swing-out Condenser 1-100x
  • Achromat Swing-out Condenser 2-100x
  • X LWD Condenser
  • D-CUO DIC Condenser Oil
  • FN-C LWD Condenser NA 0.78
    (for DIC and oblique light illumination)
  • NI-CUD Universal Condenser Dry
  • C-C Abbe Condenser NA 0.9
  • C-C Achromat Condenser NA 0.9
  • Darkfield Condenser (Oil)
  • Darkfield Condenser (Dry)
  • C-C Achromat / Aplanat Condenser NA 1.4
  • C-C Slide Achromat Condenser 2-100x
  • C-C Achromat Swing-out Condenser 1-100x
  • C-C Phase Contrast Turret Condenser*3
  • Achromat Swing-out Condenser 2-100x
  • X LWD Condenser
  • D-CUO DIC Condenser Oil
Epi-fluorescence
illuminator
Filter cube turret6 filter cubes mountable, High S/N noise terminator mechanism for all turrets
  • NI-FLT6-E Motorized Epi-fluorescence Cube Turret
    Motorized shutter, Status check function*4
  • NI-FLT6-I Intelligent Epi-fluorescence Cube Turret
    Manual shutter, Status check function*4
  • NI-FLT6 Epi-fluorescence Cube Turret
    Manual shutter
Light distribution device
  • NI-FLEI Epi-fluorescence Attachment
    Aperture diaphragm and field diaphragm (Centerable/Detachable), ND filters (ND4, ND8, ND16)
  • NI-PAU Ni Photoactivation Unit (405 nm to 650 nm lasers)
Option
  • NI-BAW-E Motorized Barrier Filter Wheel
    7 filters mountable, 0.2 sec between adjacent positions
  • NI-EXW-E Motorized Excitation Filter Wheel
    8 filters mountable, 0.15 sec between adjacent positions
  • NI-SH-E Motorized Shutter
    ?0.012 sec between open and close state
  • NI-SH-E Motorized Shutter
    0.012 sec between open and close state

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