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NIKON

ECLIPSE FN1

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Fixed Stage Microscope for Electrophysiological Research

ECLIPSE FN1

 

  • One lens covers from low to high magnifications
  • Image deeper areas with ultimate clarity with IR-DIC
  • Easy electrode placement
  • Safe, easy objective changeover
  • Operational within your reach
  • Enhanced noise reduction
  • Responsive to a broad range of experimental needs

Visualizes minute details within thick specimens with perfect clarity in patch-clamp experiments


Configuration with Narishige equipment

The ECLIPSE FN1–developed especially for electrophysiological research–features enhanced operability in every aspect to facilitate patch-clamp experiments. Advantages offered by the FN1 include slim body, streamlined structure, improved electrode placement, long working distance, and greater noise reduction. A deeper area of the specimen can be observed clearly with IR light.

  

 

 

 

 

 

 

One lens covers from low to high magnifications

Variable magnification double port

The newly developed high NA, long working distance 16x objective (NA 0.8, W.D. 3.0) provides 5.6x, 16x, 32x and 64x magnifications when combined with an optional magnification module. This objective enables observation from a low magnification widefield of up to 2.0mm to a high magnification high resolution field without changing objectives.


5.6x


32x


64x

Image deeper areas with ultimate clarity with IR-DIC

Axial chromatic aberration in near-infrared region (up to 850nm) has been corrected in 40x and 60x objectives to clearly observe the minute structure of a thick specimen. The 100x objective (NA 1.1, W.D. 2.5) is the world's first water dipping lens with depth-induced aberration correction. Because of its special correction ring, this lens can correct spherical aberration induced by imaging deep in tissue or by working at physiological temperatures. Consequently, it is ideal for IR-DIC imaging and confocal applications as well as Multi-Photon imaging. Alternating the wavelength from visible to infrared or the illumination technique from DIC to Oblique Light is carried out simply by rotating the condenser turrets.

Easy electrode placement

Access of microelectrodes to the specimen is easy as the objectives have a long W.D. of 2.5-3.5mm and broad approach angle up to 45°.

Safe, easy objective changeover

Objective up/down lever
Objective retraction mechanism

Objectives mounted on the sliding nosepiece can be raised when switching magnifications. This prevents the objective from colliding with the manipulator or the chamber. The retraction distance is 15mm, so even a thick glass dish is protected. The lens top can be easily dipped (approximately 1mm) into the bath solution by using the lever to eliminate the risk of specimen disturbance.

Operational within your reach

The focus knob and field diaphragm ring are located on the front part of the microscope base, and there are no cumbersome outside belts; so operation is easy when using a fixed stage. An optional remote handle is also available to enable operation from outside the cage.

Enhanced noise reduction

Electrical noise has been successfully reduced by utilizing fiber illumination to bring light into the system from outside the cage and by connecting ground pins to all main parts of the microscope. Vibration noise has also been reduced by undertaking critical measurement and simulation analysis to improve microscope rigidity.

Responsive to a broad range of experimental needs

By inserting a spacer between the body and the arm, you can raise the microscope height 10-30mm. This facilitates large specimen observation. Moreover, the condenser, sub-stage and turret can be removed entirely from the microscope to allow for more free space, depending on the purpose of the experiments. 

 

 

 

 

 

 

 

Optical systemCFI60 and CFI75 infinity optical system
Main bodyI-shaped, external power supply
FocusingVia nosepiece up/down movement
Manual coaxial coarse/fine focus knobs (on both sides)
NosepieceFN-S2N Sliding Nosepiece (for CFI60 objectives)
Front/back 2-position; DIC prism attachable
FN-MN-N Single Objective Holder (for CFI75 objective)
1-position; DIC prism attachable
Objective lensesCFI Plan 4x (NA: 0.10, WD: 30.0), auxiliary lens is required
CFI Plan Fluor 10xW (NA: 0.30, WD: 3.5)
CFI75 LWD 16xW (NA: 0.80, WD: 3.0)
CFI Apo 40xW NIR (NA: 0.80, WD: 3.5)
CFI Apo 60xW NIR (NA: 1.00, WD: 2.8)
CFI Plan 100xW (NA: 1.10, WD: 2.5), with correction ring
LWD condenserUniversal turret type
NA: 0.78 W.D.: 7.2mm
DIC and Oblique Light observations possible
Eyepiece10x, F.N.: 22
Eyepiece tubesC-TE2 Ergonomic Binocular Tube (Bino 100%, Bino : DSC port = 50 : 50)
(DSC port cannot be used with variable magnification double port.)
C-TF Trinocular Tube F (Bino : Photo = 100 : 0, 0 : 100)
C-TT Trinocular Tube T (Bino : Photo = 100 : 0, 20 : 80, 0 : 100)
LV-TI3 Trinocular Tube ESD (Bino : Photo = 100 : 0, 0 : 100)
LV-TT2 Tilting Trinocular Tube (Bino : Photo = 100 : 0, 20 : 80, 0 : 100)
StageFN-3PS2 FN1 Rectangular Stage (3-plate mechanical stage)
Stroke: 30mm (X, Y)
Light sourceIntensilight HG precentered fiber illuminator: 130W long-life mercury lamp
HG lamphouse: 100W mercury lamp
FN-LH Pre-centered Lamphouse: 12V-100W long-life halogen lamp
LS-DWL-N Optical Fiber Illuminator (Sumita Optical Glass, Inc.): 12V-100W halogen lamp
Operating conditionsTemperature: +10ºC to +40ºC
Humidity: 85%RH max. (no condensation)
Dimensions and weight214 (W) x 346 (H) x 422 (D)mm, approx. 12kg
(Main body)

 

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