Research Stereo Microscope
SMZ25/SMZ18
Nikon has developed an all-new stereo microscope that features a large zoom ratio of 25:1, high resolution and exceptional fluorescence transmission capability to respond to increasing demand for imaging systems that span spatial scales from single cells to whole organisms.
SMZ25
Motorized zoom model with the highest zoom ratio and resolution in the SMZ series
SMZ18
Manual zoom model providing advanced optical performance and incredibly bright fluorescence at an economical cost
| SMZ25 | SMZ18 | |
|---|---|---|
| Type | Motorized zoom | Manual zoom |
| Zooming observation | BF/DF/FL/Simple polarizing | BF/DF/FL/Simple polarizing |
| Zoom ratio | 25:1 | 18:1 |
| Magnification range | 0.63x - 15.75x | 0.75x - 13.5x |
| Maximum magnification | 315x*1 | 270x*1 |
| Maximum FOV | ø70mm*2 | ø59mm*2 |
| Maximum NA of objective | 0.312*3 | 0.3*3 |
An innovative optical system known as Perfect Zoom System provides the world's first zoom ratio of 25:1 (zoom range: 0.63x - 15.75x). Even with a 1x objective lens, the SMZ25 captures the entire 35mm dish and simultaneously delivers microscopic details.
A breakthrough in stereoscope design, Perfect Zoom System dynamically change the distance between the two optical axes as the zoom factor is changed. This change in optical axis distance enables maximization of light entry into the optical system at every magnification. The result is an uncompromised, large zoom range, high resolution in both eye paths, and minimal aberrations over the entire zoom-range. Furthermore, this breakthrough in optical design enables all of these desirable features be housed in a compact zoom body, resulting in an ergonomic instrument design.
SHR Plan Apo series
Nikon's newly developed objective lens series, the SHR Plan Apo series, offers a high resolution of 1100LP/mm (Observed value, using SHR Plan Apo 2x at maximum zoom). The new SHR Plan Apo series of lenses deliver brilliant images with true-to-life colors.
SMZ25
Conventional model
ALZ automatically adjusts the zoom factor to maintain the same field of view when switching objective lenses. This function enables seamless switching between whole organism imaging at low magnifications and detailed imaging at high magnifications.
The SMZ25 series is the first stereo microscope in the world to use a fly-eye lens on an epi-fluorescence attachment. This innovative design ensures bright and uniform illumination even at low magnifications, resulting in uncompromised uniformity in brightness across a large field of view.
Nikon's newly developed optical system offers a drastic improvement in S/N ratio even at high magnifications. This improved S/N ratio makes it possible to capture cell division, which is difficult using conventional stereo microscopes, and samples with low excitation light.
Nikon has succeeded in improving the signal and reducing noise in fluorescent images by using a short wavelength, high transmission lens. Combined with an innovative epi-fluorescence attachment, the SMZ18/25 is better able to detect excitation light than conventional fluorescent stereo microscopes.
Industrial
Bioscience
Single fluorescent neurons can be visualized in live C. elegans
Fluorescence and OCC images of a liveC. elegans expressing GFP- and RFP-neurons
(using SHR Plan Apo 2x at zoom magnification of 3x with SMZ25)
Image courtesy of Julie C. Canman, Ph.D., Columbia University
Easily obtain the information you need, such as Z drive position, zoom factor, objective lens, filter cube, and LED DIA brightness by using the Digital Sight series and NIS-Elements or Digital Sight series DS-L3 together with the microscope.
One software for all systems: NIS-Elements which is Nikon's flagship, cross-platform imaging software can now be used with Nikon's latest stereomicroscope systems SMZ25 and SMZ18. NIS-Elements enables a wide range of advanced digital imaging capabilities, easily from a PC.
Multiple fluorescent channels can be captured in conjunction with other imaging methods such as OCC or brightfield.
Individual cells resolved in a live drosophila embryo expressing GFP and mCherry
(Using SHR Plan Apo 2x at zoom magnification of 8x with SMZ25)
Image courtesy of Max V. Staller, Ph.D., Clarissa Scholes, and Angela DePace, Ph.D., Harvard Medical School
Easily setup a time-lapse imaging experiment with NIS-Elements.
(Using SHR Plan Apo 2x at zoom magnification of 9x with SMZ25 and camera head DS-Qi1)
Image courtesy of Joe Fetcho, Ph.D., Cornell University
Calcium-imaging: Time-lapse imaging of GCaMP expressing neurons inside a live zebrafish shows individual neurons firing at different times (arrowheads). The last time-frame shows a whole cluster of neurons firing (asterisk).
Capture multiple high resolution images at different focal depths to create a single extended depth of focus image or quasi-3D image.
Select the in-focus area (white square) and produces one all-in-focus image
Zebrafish embryo
(Using SHR Plan Apo 2x at zoom magnification of 3.4x with SMZ25)
Image courtesy of Hisaya Kakinuma, Ph.D., Laboratory for Developmental Gene Regulation, Developmental Brain Science Group, RIKEN Brain Science Institute
DS-L3 is an easy-to-use high-definition, large touch-panel monitor that can be used to quickly capture images without a PC or monitor. The scale bar automatically adjusts to accommodate changes in magnification. Optimal imaging parameters for each sample type and observation method can easily be set using the icons.
| SMZ25 | SMZ18 | |||
|---|---|---|---|---|
|
| |||
| DS-L3 | NIS-Elements | DS-L3 | NIS-Elements | |
| Zoom magnification |  |  | | |
| Focusing | | | — | — |
| Objective (with nosepiece) | | | | |
| Diascopic LED illumination stand (ON/OFF, light intensity control) | | | | |
| Fluorescence illuminator (light intensity control) | | | | |
| Filter cube changeover | | | | |
For other combination, please confirm Nikon.
The new LED DIA Base with a built-in OCC illuminator generates minimal heat, consumes little power and is long-life. This illuminator can enhance the contrast of uneven surfaces, such as that of an embryo.
Zebrafish embryo (using SHR Plan Apo 1x at zoom magnification of 5x with SMZ18)
Image courtesy of Junichi Nakai, Ph.D. Saitama University Brain science Institute
The OCC illuminator can be controlled using a slide lever. Thanks to scales on the slide lever, the user can save and reproduce desired illumination levels. In addition, an OCC plate can be inserted into the illumination unit from the front and rear sides, so images with different shadow direction can be observed.
What is OCC illumination?
The acronym OCC stands for oblique coherent contrast (OCC), which is a form of oblique lighting method developed by Nikon. Compared to conventional diascopic illumination that illuminates directly from below, OCC illumination applies coherent light to samples in a diagonal direction, giving contrast to colorless and transparent sample structures.
The all new remote controller provides easy access to zoom and focus controls and is designed for both right and left hand use. The remote controller contains an LCD monitor with an adjustable backlight which provides information regarding the zoom factor, objective lens, filter cube, and LED DIA brightness at a glance.
Easily switch between stereo position (stereoscopic view) and mono position (on-axis view) when using the P2-RNI2 Intelligent Nosepiece by simply sliding the objective le
Drosophila embryo dividing timelapse (Epi-fluorescence observation)
(Using SHR Plan Apo 2x, zoom magnification of 8x with SMZ25, GFP; 2.4 hrs total; imaged every 30 secs)
Images courtesy of Max V. Staller, Clarissa Scholes, and Dr. Angela DePace, Harvard Medical School
Single neurons are resolved (in green) above the OCC illumination background. (Using SHR Plan Apo2x, zoom magnification at 3x with SMZ25, GFP and OCC)
Image courtesy of Dr. Julie C. Canman, Columbia University
(Using SHR Plan Apo 2x, at zoom magnification of 6x, with SMZ18)
Image courtesy of Junichi Nakai, Ph.D. Saitama University Brain science Institute
The spindle appearing in cell division can be observed
Fertilized mouse egg, Green: Spindle (EGFP- α tubulin), Red: Nucleus (Histone H2B-mRFP1)
(Using SHR Plan Apo 1x at zoom magnification of 13.5x with SMZ25)
Image courtesy of Kazuo Yamagata, Ph.D., Center for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University
Individual olfactory nerve cells in a drosophila expressing a GFP-membrane marker are clearly resolved as black bodies encircled by fluorescent membranes (see circled area). This image demonstrates the SMZ25's incredible high resolution as the olfactory cells are typically only ø5μm in diameter
Drosphila brain, GFP-G
(Using SHR Plan Apo 2x at zoom magnification of 15.75x with SMZ25)
Image courtesy of Hokuto Kazama, Ph.D., Laboratory for Circuit Mechanisms of Sensory Perception RIKEN
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